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KMID : 1005220120270030149
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Journal of Embryo Transfer
2012 Volume.27 No. 3 p.149 ~ p.154
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In Vitro Production of Jeju Black Cattle Cloned Embryos by Somatic Cell Nuclear Transfer (SCNT)
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Kim Dong-Hoon
Yang Byoung-Chul
Im Gi-Sun
Yoo Jae-Gyu
No Jin-Gu
Park Jong-Ju
Lee Sung-Soo
Ko Moon-Suck
Park Jin-Ki
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Abstract
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This study was carried out to investigate effective condition for producing somatic cell nuclear transfer (SCNT) embryos of Jeju native cattle. As donor cells for SCNT, ear skin cells from Jeju native cattle were used. In experiment 1, the effect of recipient oocyte sources on the development of Jeju native cattle SCNT embryos were examined. Fusion rate of recipient oocyte and donor cell was not different between the Hanwoo and Holstein recipient oocytes (86.0% vs 89.9%). The rate of embryos developing to the blastocyst stage was significantly (p<0.05) higher in Hanwoo recipient oocytes than in Holstein recipient ones (28.2% vs 14.7%). Blastocysts derived from Hanwoo recipient oocytes contained higher numbers of total cells than those derived from Holstein ones (115.1 ± 40.8 vs 101.4 ± 33.3), although there were no significant difference. The mean proportion of apoptotic cells in blastocyst was not different between the sources of recipient oocytes. In experiment 2, the development of Jeju native cattle and Hanwoo SCNT embryos were compared. Hanwoo oocytes were used as the recipient oocytes. Fusion rate was not different between the Jeju native cattle and Hanwoo SCNT embryos (92.1% vs 92.9%). The blastocyst rate of SCNT embryos was significantly (p<0.05) lower in Jeju native cattle than in Hanwoo (16.9% vs 31.0%). Blastocysts derived from Jeju native cattle SCNT embryos contained smaller numbers of total cells than those derived from Hanwoo ones (136.6
± 33.7 vs 149.9 ± 39.7), but there were no significant difference. The mean proportion of apoptotic cells in blastocyst was not different between the Jeju native cattle and Hanwoo SCNT embryos. The present study demonstrated that Hanwoo recipient oocytes were more effective in supporting production of Jeju native cattle SCNT embryos, although Jeju native cattle SCNT embryos showed reduced developmental capacity when compared to Hanwoo SCNT embryos.
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KEYWORD
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somatic cell nuclear transfer (SCNT) , jeju native cattle , recipient oocyte
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